Welsh bone marrow Donor Registry

Allelic Typing Systems

Alleles detected by PCR-SSP low-resolution HLA-A typing : HLA-B typing : HLA-Cw typing
Alleles differentiated using PCR-SSP high-resolution HLA Class I typing
Alleles detected by PCR-SSP low-resolution DRB and DQB1 typing : DQA1 typing
Alleles differentiated using PCR-SSP high-resolution HLA Class II typing
Supplementary typing sets : Footnote describing use of a,b etc in given allelic ranges
References

ALLELES IDENTIFIED BY MOLECULAR TYPING - JANUARY 2003

The PCR-SSP typing sets developed at our laboratory identify HLA Class I and Class II alleles recognized by the WHO Nomenclature Committee in 2000 (5). Some additional HLA alleles have been included that were recognized following these nomenclature reports. Typing is available at high- or low-resolution. The low-resolution typing sets are able to define allele groups that generally correspond to serological specificities, whereas high-resolution typing sets are generally able to define individual alleles within the allele groups. We regularly update our typing sets to identify new alleles when sequences become available.

Sequence-based typing (SBT) is available for HLA-A, -B, -DRB1 and -DPB1 genes.


1. Alleles detected by PCR-SSP low-resolution HLA-A typing:


  1.  A*0101-0104/0106-0109 a
   2.  A*0201-0222/0224-0255 a,b
  3.  A*0301-0309  
  4.  A*1101-1113   
  5.  A*2301-2308N 
  6.  A*2402/2404-2409/2411/2413-2415/-
       A*2417-2432/2434-2436N   a,b
   7.  A*2403/2410/2416/2418/2422/2433
  8.  A*2501-2504
  9.  A*2601-2618 b 
 10. A*2901-2906
11. A*3001-3004/3006-3012 a

a,b etc see notes


12. A*3101-3106 a,b
13. A*3201-3207
14. A*3301/3303-3306 a 
15. A*3401-3404
16. A*3601-3602
17. A*4301
18. A*6601-6604 
19. A*6801-6821 b
20. A*6901
21. A*7401-7408
22. A*8001

2. Alleles detected by PCR-SSP low-resolution HLA-B typing:

  1. B*0702-0730 a,b
  2. B*0801-0815
  3. B*1301-1304/1306-1309 a
  4. B*1401-1406
  5. B*1501-1540/1542-1571a,f
  6. B*1801-1815/1817N
  7. B*2701-2711/2713-2721/2723-2725 a,b
  8. B*3501-3525/3527-3541
  9. B*3701-3705
10. B*3801-3808
11. B*3901-3920/3922-3926 a,b
12. B*4001-4016/4018-4040/4042 a,g
13. B*4101-4106
14. B*4201-4202/4204 a
15. B*4402-4431 a
16. B*4501-4505
17. B*4601-4602
18. B*4701-4704
19. B*4801-4807

a,b etc see notes
20. B*4901-4903
21. B*5001/5004 a
 22. B*5002
23. B*5101-5109/5111/5112/5114/
       5116-5123/ 5126-5129 a
24. B*5201-5203
25. B*5301-5308
26. B*5401-5402
27. B*5501-5505/5507-5512 a
 28. B*5601-5608
29. B*5701-5709 b
 30. B*5801/5802/5804-5806 a
 31. B*5901
32. B*6701/6702
33. B*7301
34. B*7801-7805
35. B*8101
36. B*8201-8202
37. B*8301

3. Alleles detected by PCR-SSP low-resolution HLA-Cw typing

  1. Cw*0102-0105 a,b
  2. Cw*0202-0205 a,b
  3. Cw*0302/0304-0310/0314/0315
  4. Cw*0303/0311-0313
  5. Cw*0401/0403-0409N a,b
  6. Cw*0501-0504
  7. Cw*0602-0607 b
  8. Cw*0701-0716
  9. Cw*0801-0809
10. Cw*1202/1208 a
11. Cw*1203/1206/1207


a,b etc see notes


12. Cw*12042/1205
13. Cw*1402 a
14. Cw*1403-1404
 15. Cw*1502-1511 a,b
 16. Cw*1505
17. Cw*1601
18. Cw*1602 a
 19. Cw*1604
20. Cw*1701-1703
21. Cw*1801/1802

(Page Index)

4. Alleles differentiated using PCR-SSP high-resolution HLA Class I typing:
Typing Sets:

Alleles differentiated
  A*02 typing
  A*32 typing
  B*15 typing
  B*27 typing
  Cw* typing
  B*44 typing

All A*02 alleles listed in the 2000 nomenclature report (5)
All A*32 alleles listed in the 2000 nomenclature report (5)
All B*15 alleles listed in the 2000 nomenclature report (5)
All B*27 alleles listed in the 2000 nomenclature report (5)
All Cw* alleles listed in the 2000 nomenclature report (5)
Under development

  High resolution typing by sequence-based typing is available for HLA-A and B genes.

(Page Index)

5. Alleles detected by PCR-SSP low-resolution DRB and DQB1 typing:


  1. DRB1*0101/0102/0104-0108
  2. DRB1*0103
  3. DRB1*1501-1513
  4. DRB1*1601-1605/1607-1608 c
  5. DRB1*0301-0321 b
  6. DRB1*0401-0443
  7. DRB1*1101-1143 b
  8. DRB1*1201-1208 c
  9. DRB1*1301-1349 b
10. DRB1*1401-1436/1438-1442
11. DRB1*0701/0703-0706 c
12. DRB1*0801-0824 c
13. DRB1*09012/0902 c


a,b etc see notes


14. DRB1*1001
15. DRB3*01-03
16. DRB4*01-03
17. DRB5*01-02
18. DQB1*0501-0504
19. DQB1*0601-0618 b
20. DQB1*0603/0606-0608
21. DQB1*0201-0203
22. DQB1*0301/0304/0309/0310/0312/0313 c
23. DQB1*0302/0307/0308/0311
24. DQB1*0303/0306 c
 25. DQB1*0305
26. DQB1*0401/0402

6. Alleles detected by PCR-SSP low-resolution DQA1 typing:
1. DQA1*0101
2. DQA1*0102/0106
3. DQA1*0103
4. DQA1*0104/0105
5. DQA1*0201

a,b etc see notes 		6. DQA1*0301/0302/0303 c
7. DQA1*0401
8. DQA1*0501-0505 c
 9. DQA1*0601

7. Alleles differentiated using PCR-SSP high-resolution HLA Class II typing:


All DRB1 alleles listed in the 2000 nomenclature report (5) can be differentiated, in the following allele groups: DRB1*01, DRB1*15, DRB1*16, DRB1*03, DRB1*04, DRB1*11, DRB1*12, DRB1*06, DRB1*07, DRB1*08; except in certain specific heterozygous combinations. Some low frequency alleles may not be differentiated from other low frequency alleles.

All DRB3, 4, and 5 alleles listed in the 2000 nomenclature report (5) can be differentiated.

All DQB1 alleles listed in the 2000 nomenclature report (5) can be differentiated e.

All DPA1 alleles listed in the 2000 nomenclature report (5) can be differentiated.

All DPB1 alleles listed in the 2000 nomenclature report (5) can be differentiated by SBT/PCR-SSP (see below).

High resolution typing by sequence-based typing/PCR-SSP is available for HLA-DRB1 and -DPB1 genes.

(Page Index)


8. Supplementary typing sets:

Typing Sets:   Alleles detected

 HPA-1 typing
 HPA-2 typing
 HPA-3 typing
 HPA-4 typing
 HPA-5 typing
 HPA-6 typing
 HFE typing
 HA-1 typing
 HA-2 typing
 ABO groups


MICA


MICB

HPA-1a, HPA-1b
HPA-2a, HPA-2b
HPA-3a, HPA-3b
HPA-4a, HPA-4b
HPA-5a, HPA-5b
HPA-6a, HPA-6b
Defines H63D and C282Y mutations
HA-1H, HA-1R
HA-1V, HA-1M (undergoing validation)
ABO alleles: O01-34, A101-106, A201-204, A301-02, Ax01-07, Ael01-02, A111, B101-107, B301-02, B(A)01, Bx01, Bel01-02, Bw01-08, CisAB01-03.

All alleles listed in the 2002 nomenclature report (6) can be differentiated (undergoing validation).

All known alleles (names not formally recognized) can be differentiated (undergoing validation).

Notes

Symbol
Interpretation
a

A*0105N (6),  A*0223 (4),  A*2401 (2),  A*2412 (3),  A*2416 (6),  A*3005 (3), A*31011 (3),  A*3302 (3),  B*0701 (2),  B*1305 (4),  B*1541 (4),  B*27051(2),  B*2722 (6),  B*39012 (3),  B*3921,  B*4017 (4),  B*4041 (6),  B*4203 (4),  B*4401 (1),  B*5003 (4),  B*5125,  B*5506 (4),  B*5803 (4),  B*7901 (3),  Cw*0101 (3), Cw*0201 (3),  Cw*0301 (2),  Cw*0402,  Cw*0601 (3),  Cw*1101 (3),  Cw*1201 (3),  Cw*1301 (6),  Cw*1401 (3),  Cw*1501 (3),  Cw*1603 (3),  Cw16042 (4) and Cw1605 (4) have been withdrawn or renamed.
b These alleles groups may be reported in various allele combinations.
c DRB1*0702 (1), DRB1*08031 (4) DRB1*09011 (3), DRB1*12031 (3), DRB1*1606 (3), DQB1*03031(3), DRB5*0201 (4), DQA1*03012 (3), DQA1*05013 (4) have been withdrawn or renamed.
d Including alleles distinguished by non-coding differences.
e Excluding alleles distinguished by non-coding differences.
f HLA-B15 alleles will be reported in various allele combinations that broadly define the B62, B63, B75, B76, B77, B70, B71 and B72 specificities.
g HLA-B40 alleles will be reported in various allele combinations that broadly define the B60 and B61 specificities.

(Page Index)
  References:
1. Bodmer JG, Marsh SGE, Albert ED, et al. Nomenclature for factors of the HLA system, 1994. Tissue Antigens 1994: 43: 1-18.
2. Bodmer JG, Marsh SGE, Albert ED, et al. Nomenclature for factors of the HLA system, 1995. Tissue Antigens 1995: 46: 1-18.
3. Bodmer JG, Marsh SGE, Albert ED, et al. Nomenclature for factors of the HLA system, 1996. Tissue Antigens 1997: 49: 297-321.
4. Bodmer JG, Marsh SGE, Albert ED, et al. Nomenclature for factors of the HLA system, 1998. Tissue Antigens 1999: 53: 407-446.
5. Marsh SGE, Bodmer JG, Albert ED, et al. Nomenclature for factors of the HLA system, 2000. Tissue Antigens 2001: 57: 236-283.
6. Albert ED, Bodmer WF, Bontrop RE, et al. Nomenclature for factors of the HLA system, 2002. Tissue Antigens 2002: 60: 407-64.

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